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Cloning by Subtractive Hybridization 629 пSTEP 1 121212 CUT Demo trading option NI RESTRICTION ENZYME 1 пппппппппппппппппппFragment 1A Fragment 1C пппппппProbe 1 Use probe 1 to isolate fragment 1A Fragment 1B пппMake probe 2 Probe 2 пSTEP 2 121212 CUT WITH RESTRICTION ENZYME 2 пппппппппппппппппппFragment 2A Fragment 2C пппппFragment 2B пппппSTEP 3 Probe 2 Use probe 2 to isolate fragment 2B Make probe 3 Probe 3 пA) Cut with restriction enzyme 1 B) Use probe 3 to isolate fragment 1B C) make probe 4 Probe 4 ппппп LECTURE 15.

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